Usefulness of the Thioglycerol as an Activator of CK (creatine kinase) in a Stable Liquid-Type Reagent

Abstract

We found thioglycerol to be stable activator for CK, and can replace NAC as a CK assay reagent. Thioglycerol was more stable than NAC, and did not produce oxidizing substances which inhibit CK activity. Thus, it is preferable to formulate a stable reagent with a longer shelf life. CK values in human serum samples tested with the thioglycerol-activated reagent agreed with the JSCC consensus method (automated method). The thioglycerol-activated CK assay was suitable for a stable liquid-type reagent.