Abstract
The conventional bromcresol purple (BCP) method of quantitatively measuring serum albumin shows false negative results when there is interference from the sulfhydryl (SH) group in serum. To eliminate this interference, we developed a two-reagent system to measure serum albumin. Serum is pretreated with the first reagent which contains sodium dodecyl sulfate and 5, 5'-dithiobis (2-nitrobenzoic acid). Then, BCP in the second reagent develops color depending on the concentration of serum albumin. Using this new system, the SH group in serum was oxidized during the pretreatment with the first reagent and the interference was eliminated. Furthermore, the high specificity, which is one of features of BCP methods, was maintained. Good precision, good linearity, and good correlation with immunological methods were observed. This new method is more accurate than the currently used BCP methods, and thus, useful for the measurement of serum albumin.
