High-molecular-mass intestinal alkaline phosphatase affects the fraction amount of bone alkaline phosphatase measured by agarose gel electrophoresis

Abstract

We demonstrated the serum sample treated with neuraminidase and protease by agarose gel electrophoresis, and calculated the activity of bone-type alkaline phosphatase (BAP) fraction. From the result, high-molecular-mass intestinal alkaline phosphatase (HIAP) takes place to overlapping with the activity of BAP fraction. The protease-treated and non-treated samples were both subjected to agarose gel electrophoresis. Data showed a decrease in BAP in the α₂β region and increase in normal-molecular-mass intestinal alkaline phosphatase in the β region were as HIAP positive. Therefore, the serum sample used for the isozyme measurement on the agalose gel electrophoresis, the protease treatment may be essential. Resulting that a good correlation (r=0.982) was found between the BAP activity determined by the mentioned-above electrophoresis method using a protease treatment, and the BAP activity determined by the ELISA method.