Abstract
In the urine of subjects given an oral dose of spironolactone, several metabolites have been found. Metabolites which interfere with the estimation of 17-oxosteroids (17-KS) by the zimmermann reaction and 17-hydroxycorticosteroids (17-OHCS) by the Porter- Silber reaction were analyzed by thin-layer chromatography, and the interference of each metabolite with the estimations of 17-KS and 17-OHCS was investigated. In the estimation of 17-KS, the metabolite of spironolactone had a maximum absorption at 610nm. The values obtained by the formula of Fraser give positive errors, but the values are corrected by using the reference absorption. In the measurement of 17-OHCS, since the interference is caused by the reaction of the metabolites with sulfuric acid, the value is corrected by subtracting the blank value.
