Electroplloretical Separation of Amylase Isoenzymes

A Comparison of Methods on Cellulose Acetate Membrane and Agar Gel Electrophoresis

Abstract

acetate membrane as supporting medium and Blue-Starch® as staining material was carried out. This technique could be applied as a routine laboratory procedure because of its simpilcity and reproducibility.
The pattern of migration of isoenzymes in the this method is in good agreement with that of classical method based on agar gel separation. Futhermore, in some particular instances, the separation of isoenzymes with the this method is better than that with agar gel.