An Assay Method of Glucose-Forming Amylase in Human Urine

Abstract

The glucose-forming amylase in human urine was assayed simply and accurately without interference of α-amylase, proteinous materials, and reducing substances by the method based on the following principles: A small portion of dialyzed urine was incubated with maltotriitol as the substrate, the enzyme reaction was stopped by adding acid, and the glucose liberated was determined by the Park and Johnson method after neutralizing and deproteinizing the incubation mixture by the centrifugal gel filtration method.
The present paper describes the assay method definitely, presenting a number of experimental data obtained for urine from many healthy Japanese male adults. Also, some discussions are made about the reason of employing maltotriitol as the substrate and Park and Johnson method as the glucose determination method.