Effects of Protein on the Determination of α-Amylase Activity in Urine

Abstract

Effects of albumin or high molecure compound on α-amylas eactivity by use of Blue Starch Method has been widely reported. Rate of increased activity by addition of albumin was varied from 10 to 90 percent, using urine samples which are protein negative by sulfosalicyli cacid method. To investigate the cause of change in activity, analysis of chemicals, α-amylase isozyme and trace amount of protein in sample was done. On as tudy of trace amount of protein not detected by sulfosalicylic acid method, amount of protein presented in urine is in proportion to the rate of increased α-amylase activity. These results show that the certain amount of albumin for the determination of α-amylase activity in urine or pancreatic juice required to obtain a constant result in routine test.