Rapid and Microdetermination of Carbon Monoxide in Blood using Oxygen Electrode and Light Irradiation Apparatus
1978 Volume 7 Issue 2 Pages 177-182
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1978 Volume 7 Issue 2 Pages 177-182
The authors deviced a rapid microdetermination carbon monoxide (CO) in blood using polarographic oxygen (O2) electrode and light irradiation apparatus. This method consists of following procedures; (1) 15μl of the CO-biood was aded into 1 ml of O2-depleted water (O2: 4-5 μg/ml) in the closed glass cell fixed O2 electrode. By this step, deoxyhemoglobin (HHb) in the CO-blood was saturated with dissolved O2 in the O2-depleted water to become oxyhemoglobin (HbO2). The amount (a) of oxygen corresponding to [HbO2 (a') formed from HHb and HbO2 (a') already existed] in the sample was recorded by addition of 10 μl of 10% K3Fe (CN)6.(2) The same CO-blood diluted with water was bubbled by O2 gas under light irradiation. By this procedure, HHb and HbCO in the CO blood were completely changed into HbO2 The quantity (b) of O2 combined with total Hb was determined by addition of 10 μl of 10% K3Fe (CN)6, successively. The value, [(b-a)/b] ×100, indicates the per cent of HbCO on the total Hb of the CO-blood tested. This method requires only 35 μl of the CO-blood and 6-7 min for each determination. Results showed excellent agreement with those obtained by spectrophotometry (r=0.993, y=0.95× ±2.14). Within-run precision was measured from 30 blood samples containing 50.0% HbCO, and the standard deviation and coefficient of variation were ±1.3% HbCO and 2.6%, respectively.
