Enzymatic Determination of Serum Creatinine Using Creatinine de minase

Abstract

An enzymatic determination of serum creatinine using creatinine deiminase (EC 3.5.4.-) and glutamatedehydrogenase (EC 1.4.1.2) is described. The principle of this method is as follows;creatinine is degradated by creatinine deiminase to produce ammonia and N-methylhydantoin, and the ammonia produced is coupled with glutamate dehydrogenase. The change in absorbance at 340nm is mesured.
The correlation coefflcient between the present method and Folin method was 0.997 and regression line was y=1.0x-0.26 (y=present method In this method). serum creatinine is determined without deproteinization.