Abstract
A new method was devised for determination of serum steroid hormorles having the4-en-3-one structure by high Performance liquid chromatography.
The devised technique involves the gradient elution with CH2Cl2-C2H5OH-H2O (98.8:1.0:0.2 and 89.7:10.0:0.3) on a column of Zorbax Sil monitoring the absorbance at 254nm.
Thus a synthetic mixture of the structurally related hormones such as progesterone, 4-androstene-3, 17-dione, adrenosterone, deoxycorticosterone, 17α-hydroxyprogesterone, 11β- hydroxy. 4-androstene-3, 17-dione, 11-desoxycortisol, testosterone. corticosterone, cortisone, aldosterone and cortiSol, could be satisfactorily separated.
The Δ4-3-ketosteroids were evaluated from the ratio of peak area or peak height to that of prednisolone, an interrlal standard.
For the clinical purposes, serum specimenes of 6 normal male, 5 normale female were sublected to the analysis. The observed value were found in good agreement with the reference values.
