Abstract
Conditions for chemiluminescence reaction of luminol-K3Fe (CN) 6-hydrogen peroxide mixtures have been investigated using an ATP photometer.
The following procedure for the measurement of hydrogen peroxide by luminol chemiluminescencence reaction was proposed.
1) For the measurement of hydrogen peroxide in the range of concentrations between 4.4μmol/L and 88μmol/L: Mix 20μl of samples with 100μl of 0.01mmol/L luminol in a 0.1mol/L H3BO3·KOH solution (pH11.5). Immediatly after mixing, add 100μ/l of 2mmol/L K3Fe (CN) 6 to the mixture and stand for 15 sec. Then, measure integrated intensity of luminescence for 1min.
2) For the measurement of hydrogen peroxide in the range of concentrations between 8.8μmol/L and 880μmol/L: Mix 20μl of samples with 100μl of 0.2mmol/L luminol in a 0.1 mol/L H3BO3·KOH solution (pH 11.5). Immediatly after mixing, add 100μl of 4mmol/L K3Fe (CN) 6 to the mixture and stand for 15sec. Then, measure integrated intensity of luminescence for 1min. Using these procedure, the error of measurement of hydrogen peroxide were within ±2%.
