Heparin-Ca-Ni Precipitation Method for Measuring Cholesterol in High-Density Lipoprotein

A Provable Mechanism for Precipitation and Optimum Assay Condition

Abstract

Heparin-Ca precipitation method for measurement of cholesterol in high-density lipoprotein (HDL-C) has been improved for a good separation of HDL from low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) in normo-and hyperlipemic sera by adding NiCl₂ (1mM) to heparin-Ca reagent.
We proved that this method can be used for the separation of HDL fraction from the other lipoproteins in a specimen of serum triglycerides (TG) concentration of about 1g/dl.
The correlation between the results of dextran sulfate-Mg precipitation method and present method is very high (n=46, r=0.980) with regression line, y=1.05x+0.4. Precision (CV) % was found to be 2.3% (n=10) within run and 3.5% (n=20) run-to-run.
Present method was applied to measure HDL-C in normo-and hyperlipemic sera in the routine clinical measurement.