A Simple and Rapid Measurement of Glucose in Serum Using 3-Methyl-2-Benzothiazolone Hydrazone Hydrochloride and Glucose Oxidase-Catalase Enzyme System

Abstract

The 3-methyl-2-benzothiazolone hydrazone hydrochloride (MBTH) method for the assay of glucose was improved to facilitate simple, rapid and sensitive estimation of this compound in serum. In this procedure, serum can be incubated with the enzyme system in the presence of MBTH. The incubation time was remarkably shortened by the elevation of incubation temperature. The present method is suitable for the assay of glucose using diluted serum.