Abstract
The anaerobic degradations of palmitic acid was investigated by the batch experiments at 35°C and 55°C. Palmitic acid degradation rate and the maximun loading rate under thermophilic condition were 1.4 and 1.8 times higher than that under mesophilic condition, respectively. Although in thermophilic palmitic acid degradation rate was higher than that in mesophilic, acetate degradation rate was almost the same. Then methane production rate was same at mesophilic and thermophilic conditions. The number of hydrogen utilizing methanogenic bacteria in mesophilic and thermophilic sludge were 4.9×106MPN/mL and 1.7×108MPN/mL, respectively.