Abstract
Studies of energy recovery from cellulosic biomass has been widely conducted. However, to establish the utilization technologies of cellulosic biomass, there are several technical problems to overcome such as processing cost, establishment of effective pretreatment, and disposal of residue. Therefore, our research group have developed the multistage treatment process for effective utilization of cellulosic biomass to reduce the treatment cost. The process recovered a valuable resources and sugars by pretreatment and enzymatic saccharification, and methane recovered from residue of enzymatic saccharification by methane fermentation finally. In this study, effect of NaOH pretreatment for the multistage treatment process was investigated by using waste mushroom bed as a cellulosic biomass. As the result, lignin (30%, w/w), silica (38%, w/w), and sugar (25%, w/w) were eluted and recovered in 2.0%-NaOH pretreatment process. The amount of sugar and methane recovery in enzymatic saccharification and methane fermentation increased using NaOH pretreatment. Consequently, 51% (w/w) of resource was recovered from waste mushroom bed by multistage treatment process with 2.0%-NaOH pretreatment.