Abstract
Effects of nickel ion on microbial community structures under high salt concentration environment were investigated. Nickel resistant microbial cultures were enriched in a chemostat reactor by feeding landfill leachate in which nickel (II) chloride was added. Stable enrichment was established up to 2 mM of nickel (II) chloride addition. Microbial community structures of enrichment cultures were analyzed by pyrosequencing. Drastic community change, even at class level, was observed when nickel (II) chloride concentration was increased. In the culture enriched with 2 mM of nickel ion, Nitratireductor were the dominant genus, indicating that they are likely to have nickel resistance under the high salt concentration environment. Novel nickel resistant microorganisms, including the member of Nitratireductor, were isolated from the reactor.
