NOVEL METHOD FOR RECOVERY OF TARGET VIRAL RNA GENOME IN SEWAGE BY USING HYBRIDIZATION

Abstract

 Molecular analysis of pathogenic viruses is promising to evaluate the spread of infectious diseases caused by the viruses. However, current methods can analyze limited region of virus genomes, which hampers detailed virological studies of the viruses. In this study, we developed a recovery method of target viral RNA genome by using hybridization method to be coupled with metagenomic analysis. The optimized method could successfully recover the target virus genome (enteroviruses) at high recovery rate (71%), while the recovery rates of non-target viruses were low (0.19% for Pepper Mild Mottle Virus and 0.18% for Murine Norovirus, respectively). These results clearly demonstrated that the developed method could recover target viral genomes with high specificity and efficiency.