Isolation of phage-displayed-peptide-ligands specific to Shewanella algae cells from a phage display peptide library and application for magnetic cell separation

Abstract

 Peptide-ligands bind to Shewanella algae cells were selected from a random 12-mer phage display peptide library. 2 rounds of biopanning against S. algae cells were carried out to enrhich phage clones displaying the specific peptide-ligands. After the second round, eluted phages from S. algae cells were cloned and analyzed the binding specificity to the target cells by fluorescence signal detection using a Tyramide Signal Amplification. Four phage clones determined to be higher binding specificity were selected and subjected to cell separation using magnetic beads. As the results, magnetic beads with the selected phage clones could collect the target cells compared with magnetic beads without phages. Therefore, phage displayed peptide combined with magnetic beads may be an applicable technique for specific microbial cell separation.