MOLECULAR ANALYSIS OF LOTUS PRODUCTION SOIL CAUSING REPLANT PROBLEM AND EVALUATION OF FERTILIZATION EFFECT OF BACILLUS AND NON-PARASITIC NEMATODES PREDOMINATED COMPOST

Abstract

 In this study, to attempt a development of nematodes control method without chemical pesticide for lotus production field, we performed comparative analysis of microbial communities by difference of disease level, quantitative PCR of parasitic nematodes, and cultivation test of Nelumbo nucifera (lotus) production soil using Bacillus and non-parasitic nematodes predominated compost. As a result of comparative analysis of microbial communities by 16S rRNA genes sequence, microorganisms belonging to uncultured groups in phyla Acidobacteria and Chloroflexi were specifically detected in lotus production field causing replant problem. The result of qPCR targeting parasitic-nematodes indicated that parasitism of Hirschmanniella diversa and H. imamuri occurs in small part of lotus root causing disease. As an evaluation result of effect of Bacillus and non-parasitic nematodes predominated compost, we confirmed that genus Bacillus is able to grow in lotus production field fertilizing the compost.