Abstract
In this study, we monitored the stream temperature of four first class rivers in Chugoku District, Japan, from July to August 2016, and determined the environmental DNA (eDNA) concentration of Plecoglossus altivelis altivelis. We attempted to understand the relationship between the concentration of P. altivelis altivelis and the mean stream temperature. Our results showed that the longitudinal distribution pattern of P. altivelis altivelis differs along the studied rivers, and the concentration of eDNA is high in sites where the mean stream temperature from July to August remains close to 23 °C. These results indicate that P. altivelis altivelis may have a suitable stream temperature in summer.