CONTROL OF HYDROGEN SULFIDE BY PROVIDING ELECTRON PASSWAY WITH HIGHLY CONDUCTIVE CONCRETE IN SEWER PIPE AND BACTERIAL FLORA ANALYSIS OF ELECTRICITY-PRODUCING BACTERIA

Abstract

 The objective of this study is to reveal the evidence of hydrogen sulfide (H₂S) control by biological oxidation through conducting the long-term experiment of H₂S mitigation by providing electron pathway with highly conductive concrete and bacterial flora analysis of electricity-producing bacteria. The long-term experiment of H₂S mitigation by biological oxidation with rod shaped electrode (anode) of highly conductive concrete was conducted. As the results, it confirmed that H₂S was mitigated. Time-course changes of bacterial flora through this experiment were investigated by PCR-DGGE. From the results, electricity-producing bacteria (Geobacter sp. and Pelobacter sp.) were found around the inner surface of anode. From the results of quantitative analysis of electricity-producing bacteria with metagenomic analysis by next generation sequencer, it was shown that a lot of electricity-producing bacteria (Geobacter sp.) were accumulated around the inner surface of anode. Finally, the evidence of hydrogen sulfide (H₂S) control by biological oxidation (using the oxygen dissolved in surface water as electron accepter nevertheless under anaerobic condition) was revealed.