Abstract
In this paper, an in-situ permeability control method is proposed for soil contaminated sites, and its efficiency is assessed in a series of laboratory tests including cultivation tests and permeability tests. The main outcomes of this research were: (1) The source of calcium ion, carbon source and pH buffer was required for the in-situ permeability control method using microbial function. (2) The proposed method can enhance the calcium carbonate precipitation and can reduce soil permeability. (3) The calcium carbonate precipitation processes could be monitored by SEM-EDS and PCR-DGGE. An in-situ soil permeability control method can be utilized to control of contaminated groundwater. Microbial analysis using molecular techniques is effective for the monitoring and management of the calcium carbonate precipitation process and in-situ soil permeability control.
