DEVELOPMENT OF NOVEL CARACTERIZATION METHID FOR HUMIC SUBSTRANCES BY USING ULTRAFAST-SCANNING SPECTROFLUOROMETER

Abstract

 The establishment of a more detailed and simple method for clarifying humic substances components in small sample numbers was investigated by LC-EEM using an ultrafast scanning spectrofluorometer. In offline EEM-PARAFAC, the sample water level in the microplate wells could not be kept constant during EEM measurement, and any humic fractions could not be separated. On the other hand, in inline-EEM using the ultrafast scanning fluorescence spectrophotometer as a detector, it was demonstrated that the generated three-dimensional array satisfies the trilinear condition. The 275 EEM spectra obtained from river water samples collected from 11 locations were subjected to PARAFAC analysis, and the spectra were separated into four components, which enabled us to grasp the increase and decrease of humic constituents including fulvic acid, the behavior of which is difficult to grasp. By applying the developed method, it is believed that DBPs-FP can be predicted concisely and with high accuracy in the water treatment process.