Abstract
We verified an accuracy of previous investigation technique of benthos in artificial tidal flat. In addition, we studied a characteristic of benthos by an investigation depend on a division of using height and clay ratio in artificial tidal flat. As a result, (i) A species richness is increasing in artificial tidal flat, (ii) An identified the ratio of species richness was 77% by sampling using the division, on the other hand, 72% by sampling at random. Therefore the previous investigation technique of benthos is effective, (iii) A benthos of no identified in natural tidal flat was fixed on artificial tidal flat.