Abstract
Fish diversity at Amagasaki canal and port area were investigated by environmental DNA metabarcoding (MiFish method) to clarify whether this method is valuable in eutrophic conditions. The result suggests that detection of fish diversity in this study area required filtering more than 1000 ml water. The MiFish method detected 16 species, which was larger than 13 species, the number detected by the basket method in last year. The MiFish method detected Bora (striped mullet) and Acanthopagrus, which were not caught by basket methods because of the size. The number of detected species and fish fauna suggests that MiFish method is efficient in monitoring biodiversity at canal and port area. However, it must be noted that amount of filtering water should be selected, and the monitoring result should be checked carefully whether it fits well with actual fish fauna.
