Effects of lymphokines, OK-432 and indomethacin on EB-virus specific cytotoxic T lymphocyte (EBV-CTL) activity

I. The specificity and the effects on EBV-CTL activity of normal controls

Abstract

Effects of lymphokines, OK-432 and indomethacin on EB-virus specific cytotoxic T lymphocyte (EBV-CTL) activity were tested, and the results were as follows. Recombinant interleukin 2 (RIL-2, 0.2U/ml) and recombinant interferon α (RIFN-α, 500IU/ml) enhanced the EBV-CTL activity when added 18 hours before CTL assay. Recombinant interferon γ (RIFN-γ, 500IU/ml), OK-432 (0.01KE/ml) and indomethacin (1μg/ml) did not enhance the EBV-CTL activity. RIL-2 and RIFN-α did not enhance either the EBV-CTL activity against allogeneic lymphoblastoid cell-line or K-562 cells, and also did not enhance the EBV-CTL activity of seronegative individuals.
RIL-2 and RIFN-α can enhance the EBV-CTL activity specific for EB-virus and enhanced EBV-CTL should be MHC restricted activity.