Abstract
Human peripheral dendritic cell was successfully obtained with little contamination of macrophage. Dendritic cell showed a cluster formation with autologous T cells. These T cells were mainly CD-4 positive and partially blastoid. Clusters were isolated and then recultured. CD-4 positive cells were grown from isolated clusters confirmed by phase contrast microscopy and 3H-thymidine incorporation.
This cell growth was augmented by pretreatment of dendritic cell with α-interleukin 1. Cluster supernatant contained some factor to drive SAC-stimulated B cell into antibody secreting cell.
Our experiment using peripheral dendritic cell would be able to apply the clinical investigation on dendritic cell function in some patients.
