Abstract
Nine monoclonal anti-platelet autoantibody producing human cell lines were established from patients with idiopathic thrombocytopenic purpura (ITP) by Epstein-Barr virus (EBV) transformation and their characteristics were studied. Three of the antibodies showed cross-reactivity with single-stranded DNA (ssDNA), as determined by enzyme-linked immunosorbent assay (ELISA) and one of them was also found to bind to the synthetic polynucleotides, poly (dT) and poly (I), as well as to ssDNA.
One of these EBV-transformed human cell lines was fused with the mouse myeloma P3X63 Ag8.653. The resulting hybridoma could be grown in nude mouse ascites by which large quantities of human monoclonal antibodies were obtained.
