Abstract
The peroxidase-labelled IgG fraction of anti-nRNP serum obtaind from a MCTD patient was used to study the intracellular localization of n-RNP antigen. Nuclei of almost all kind of cells taken from man and rat were stained in a speckled pattern. The nuclei of the tonsil lymphocytes showed a various pattern of staining with thick and thin tone.
Cytoplasmic staining was observed in the anterior horn or rat spinal cord and this staining was abolished by treating the tissue sections with RNase for 90 min.
In the nodule of hepatocellular carcinoma, intensity of the staining by the labelled anti-nRNP antibody decreased from periperal to central zone.
During the cell differentiation of the cultured cells HeLa, the staining of nRNP antigen changed from nuclei to cytoplasma.
The presence of U1 nRNP antigen in different tissue distribution and durign cell cycle might be affect for the clinicophathology of patients with MCTD who have autoantibody to this antigen.
