Abstract
Macroscopically normal appearing fresh surgical tissues were obtained from the operated lungs and were fixed in PLP fluid or Carnoy's fluid. Samples were incubated with a murine monoclonal antitryptase antibody provided commercially for the use of RIA assay for mast cell tryptase. Immunohistochemical method with SAB showed that tryptase positive lung mast cells were stained in a brown color and these cells were predominant in alveoli. These results above suggest that the present method is an useful immunohistochemical procedure to see the possible involvement of mast cells in the pathogenesis of allergic pulmonary diseases.
