Perforin production and its regulation in IL-2 activated killer (LAK) cells

Akihiko Nii; Kohji Sugihara; Akito Nakamura; Masanobu Munekata; Yasuhiko Nishioka; Seiji Yano; Saburo Sone; Takeshi Ogura

doi:10.2177/jsci.15.712

Akihiko Nii, Kohji Sugihara, Akito Nakamura, Masanobu Munekata, Yasuhiko Nishioka, Seiji Yano, Saburo Sone, Takeshi Ogura

Author information

Akihiko Nii
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Kohji Sugihara
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Akito Nakamura
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Masanobu Munekata
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Yasuhiko Nishioka
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Seiji Yano
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Saburo Sone
The Third Department of Internal Medicine, The University of Tokushima School of Medicine
Takeshi Ogura
The Third Department of Internal Medicine, The University of Tokushima School of Medicine

Keywords: Perforin, CD 8⁺ lymphocytes, Interleukin-2, Transferrin

JOURNAL FREE ACCESS

1992 Volume 15 Issue 6 Pages 712-715

DOI https://doi.org/10.2177/jsci.15.712

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Abstract

We investigated the regulating mechanism of LAK cell induction in terms of perforin production by a semiquantitative method using universe microphotospectrometry. Only CD 8⁺ lymphocytes showed perforin production after IL-2 stimulation. Perforin productions of CD 8⁺ lymphocytes were markedly up-regulated by addition of monocytes, TNF-α, or transferrin to culture medium with IL-2. These findings indicate that up-regulation of LAK activity is due to enhanced production of perforin, and suggest that study of perforin production in cancer patients may be important in immunotherapy using IL-2 and LAK.

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