Abstract
This communication describes a successful application of two-directional rocket method to simultaneous quantitation of various substances. Differential assay of albumin and IgG, which have different mobility and antigenicity, was found easiest, by changing the agarose/agar ratio to get an appropriate electroendosmosis of the gel. That of C3PA and C3A, which have identical antigenicity but different mobility, was found likewise easy, for on addition of EDTA to agar, anodal migration of C3PA alone took place, while C3A remained in cathodal side.
Differential assay of SC and SIgA was also successful: After transferring SC from β1-region to α1-region by addition of heparin-Ca EDTA the two substances were separated in the opposite direction by changing agarose/agar ratio. The height of the rocket-shaped precipitation peaks formed in two directions was found proportional to the concentration of the antigens.
According to our postulation the effect of the polyanionic charge of heparin is a contributing factor to this effect of heparin-Ca EDTA on SC, because the electric mobility of SC was altered by other anion surfectants, and in addition, this effect was quite vulnerable to the influences of pH and ionic strength.
In using this method as a screening assay of saliva donors for detection of primary immunodeficiency, 2 cases of isolated IgA immunodeficiency and 2 cases of hypoglobulinemia were picked up from 16, 000 infants in Shida District over a 3 year period.
In view of the significant role of SIgA in local protective mechanism, this two-directional rocket method seems to be recommended, because of its easy sampling procedure.
