Gamma-globulin therapy to whooping cough II

Neutralization of leukocytosis or lymphocytosis promoting factor and histamine sensitizing factor from Bordetella pertussis by human gamma-globulins

Abstract

We assesed, using the mouse system, whether the human gamma-globulin preparations could neutralize the leukocytosis or lymphocytosis promoting factor (LPF) and histamine sensitizing factor (HSF) of crude LPF preparation extracted from the supernatant of the liquid culture of B. pertussis. Intact gammaglobulin (GG), S-sulfonated gamma-globulin (S-GG), and pepsin-treated gamma-globulin (P-GG) were used as gammaglobulin preparations in this study.
1. All gamma-globulin preparations (GG, S-GG, and P-GG) could neutralize the LPF and HSF.
2. The neutralizing activity to LPF was found even when the S-GG was administered 4 and 8 hours after the injection of crude LPF preparation. But the activity was diminished when S-GG was given 24 hours later. The neutralizing activity to HSF was found at 4 hours injection, but reduced at 8 and 24 hours injection of S-GG after the administration of crude LPF preparation.
3. All gamma-globulin preparations produced precipitin bands in counter-immunoelectrophoresis with the crude LPF preparation.
These results suggested the presence of neutralizing antibodies in the gamma-globulin preparations to LPF and HSF.