Detecting K cell Subpopuration in Patients with Various Diseases by the Microplate Method

Abstract

ADCC effector cell (K cell) can be well detectable by microplate method. We studied the distribution of K cell in the human lymphoid organs, and assesed K cell subpopulation in peripheral blood of the patients. The results are summarized as follow:
1. K cell was detected in peripheral blood and bone marrow, but not in lymphnode and thymoma.
2. K cell population of peripheral lymphocytes was 6.6±2.4% in normals.
3. K cell percentage was elevated in the patients with pulmonary tuberculosis. On the other hand, it was depressed in the patients with untreated carcinoma and malignant lymphoma.
4. No correlation was found between K cell percentage and the age or PPD skin reaction of the patients.