1984 Volume 7 Issue 4 Pages 216-223
The effect of anti-DNase I antibody on an enzymatic activity of DNase I and anti-DNase I antibody in collagen diseases were studied. Rabbit anti-DNase I antibody which was raised by immunization with bovine pancreatic DNase I was shown to be a DNase I inhibitor. Sera from 75 SLE patients, 28 RA patients, 9 PSS patients and 30 healthy individuals were examined to detect antibody to DNase I by Farr test. Radioactive iodine-labeled purified bovine pancreatic DNase I was used as an antigen in the assay. Mean antibody-value of the each group of the patients was significantly higher than that of the healthy group. The incindence of the antibody, regarded as positive when the value exceeded the mean value+2SD of the healthy group, was 56% in SLE, 50% in RA, 22% in PSS and 0% in healthy individuals. Possible roles of anti-DNase I antibody in collagen diseases were discussed.
