Abstract
For the purpose of inducing the anti-tumor effect in the portal vein, the intrasplenic serial Biological Response Modifier (BRM) administration was performed by using our original morbilized spleen subcutaneous locating rats. In a view point of endogenous Tumor Necrosis Factor (TNF) production, lipopolysaccaride, BCG, and OK 432 were chosen as administrating BRM and injected into the spleen frequently.
The study of the portal blood serum revealed that BRM administration group gained higher cytotoxic activity against L 929 cells significantly (p<0.05) than control group, which is possibly concerned with TNF. And interferon production in portal vein increased significantly (p<0.05) in BRM administration groups.
On the other hand, the study of the mononuclear cells in the portal vein and spleen after intrasplenic serial BRM administration showed higher cytotoxic activity against YAC-1 cells than control group significantly (p<0.05).
According to the result of our study, the activation of the latent immunological function of the spleen, which is the largest lymphoid system in vivo, could induce many-sided antitumor effect in the portal vein. Therefore, it is prospective that intrasplenic serial BRM administration could be new process for suppression of the portal venous implantation.
