Abstract
We examined the fundamental assay condition of 48 Well Micro Chemotaxis Chamber to mesure the monocyte chemotaxis in children. By using polycarbonate membrane filter (5 μm pore, polyvinyl pyrrolidone-free), we confirm the chemoattractant dose-dependent chemotaxis and positive correlation between the number of migrated monocytes and the number of monocytes in the upper well or the incubation time.
The chemotaxis of monocyte was not affected by the preincubation of monocyte with theophylline, salbutamol sulfate, sodium cromoglycate and prednisolone.
In the study of MCLS patients, chemotaxis index (chemotaxis to zymosan activated serum/random migration) was lower in the patients with positive CRP than in the patients with negative CRP or normal controls. And the index increased as CRP decreased.
