1986 Volume 9 Issue 6 Pages 495-501
Recent reports say that it is possible to remove MHC class I from the surface of cell membrane specifically with short termed acid treatment, as a result, LAK activity increases significantly against acid treated cells. For the purpose of introducing the effect to in vivo, the short termed acid treatment was performed to the murine peritonitis carcinomatosa with Meth A fibrosarcoma.
In vitro, 51Cr release assay revealed that LAK activity increased against acid treated Meth A cells significantly. And in vivo, it was safe that tumor-bearing mice were treated with our technique under anesthesia, so that there were no mortal cases.
On the other hand, the effect of LAK cells and recombinant IL-2 on peritonitis carcinomatosa models in mice was studied with % survival. There was no effect for survival between control group and only LAK i. p. group, but treatment with LAK cells plus acid treatment lengthened median survival significantly. Treatment with IL-2 administration and with LAK cells plus IL-2 cured these mice partially, but in these group plus acid treatment, the therapeutic effect was more evident. With the results, it is prospective that acid treatment before adoptive immunotherapy could enhance the antitumor effect for local therapy.
