Abstract
The aim of this study was to organize a laboratory exercise to study the relationship between CYP2C19 genotype and phenotype measured by omeprazole metabolism. In the Department of Clinical Pharmacology, Hirosaki University School of Medicine, 5th year medical students participated in the laboratory exercise of a 2-day course after they gave written informed consent. They were phenotyped by measuring plasma omeprazole and 5-hydoxyomeprazole concentrations after administering 2 tablets of 20 mg omeprazole, and also genotyped with a PCR-RFLP method to identify defective mutation alleles for CYP2C19, CYP2C19*2 and CYP2C19*3. The procedure of CYP2C19 genotyping and phenotyping required almost one day, but most 5th year medical students were successfully able to demonstrate the relationship between their genotype and phenotype. Although some PMs showed atypical metabolic ratios of omeprazole to 5-hydroxyomeprazole probably due to a few sampling method, our laboratory exercise led to reliable and impressive results for medical students.
