Cyclosporin A Therapy and Drug Monitoring by HPLC following Renal Transplantation

Abstract

We describe cyclosporin A therapy and drug monitoring by HPLC and RIA following renal transplantation or in a patient with Bechet's disease. There was considerable variation in the relationship between HPLC and RIA measurements. The concentrations of cyclosporin A measured by HPLC were invariably less than those measured by RIA. In concentration-time curves of cyclosporin A after dosing, the peak concentrations were observed at 1 to 2 hours in all patients. During the clinical course of patients after renal transplantation, there were considerable variations in RIA measurements, and attempts were made to achieve a concentration of from 100 to 300 ng/ ml by HPLC. During the study period, no renal toxicity or rejection episodes were observed in any patient.