Novel approaches for transporter studies

Abstract

Transport is an important factor that determines endogenous concentrations of a compound in association with biosynthesis and catabolism. However, our understanding on the molecular mechanisms for transport is limited compared to those for biosynthesis and catabolism. We recently developed a modified yeast two-hybrid system to functionally screen transporters for the plant hormone abscisic acid (ABA). Here we describe our strategy for identification of ABA transporters and discuss how we can apply the strategy to identify transporters of other compounds.