Live imaging and image analysis of organelles in plant cells(<Feature Articles>Live-cell imaging)

Abstract

Live imaging was brought about by recent development of fluorescent proteins and innovation of imaging systems. Dynamic analyses of organelle through live imaging reveal the subcellular dynamic function and the spatiotemporally four-dimensional structure. At the same time, quantitative analyses in addition to qualitative analyses become more important in analyses of the huge imaging data. Thus, we introduce our recent works in live imaging of mitochondria, nuclei, vacuolar membrane, and actin filaments in plant cells with our observation skills. Moreover, quantitative analyses of live imaging data were classified into three categories, number, morphology and distribution, three dimensional shape or dynamics. We review these three quantitative analyses.