Metabolism of jasmonic acid and transportation to reveal its own biological activities

Abstract

Plants are sessile organisms and are unable to avoid environmental biotic and abiotic stress by changing their habitat.　Therefore, plants have developed unique defensive systems.　Plants use hormones not only to control growth and development but also to initiate defense responses to these stresses.　Jasmonic acid （JA） is categorized as one of plant hormones, and we focused on metabolism, transportation, and biological activities of JA.　The unique point of this study is preparation of various kinds of labeled compounds for a target compound, and analyze them using MS/MS system, which afford following experimental results; 1】de novo synthesis of jasmonoyl isoleucine （JA-Ile）in damaged leaves and its transportation into non-damaged leaves to induce JA depended wound response, 2】metabolism of JA-Ile via CYP94b3 to give 12-hydroxyJA-Ile, 3】isolation of cis-jasmone form Lasiodiplodia theobromae and investigation of its synthetic pathway to give cis-jasmone.