Spatiotemporal plant hormone analysis using laser microdissection

Abstract

Laser microdissection (LMD), also called Laser capture microdissection (LCM), is a method for isolating different tissue cells or specific single cells from various tissue samples under direct microscopic observation. The LMD method enables isolating the cells of interest region or specific cells for several analyses such as DNA/RNA analysis, proteomics, metabolomics, and other molecular analysis. This approach can be used to study various biological events at the tissue or cellular level, and the LMD method has also been used for a wide range of purposes in the plant sciences. This paper describes a technique for spatiotemporal phytohormone analysis using LMD to isolate different tissues/specific cells from cryosections of incised flowering stems of Arabidopsis thaliana.