Abstract
Recently, chemical genetics approaches are often used in plant biology. Identification of target protein is an integral, but rate-limiting step in chemical genetics. Here we show the rapid target analysis system using the Arabidopsis full-length cDNA over-expressing (FOX) lines. When anti-malarial fosmidomycin (FSM) that causes albino phenotype on Arabidopsis wild-type plants was tested as a model, FSM-resistant mutants that over-express a gene coding target enzyme in the methylerythritol phosphate pathway was promptly isolated from the FOX lines. We also isolated FSM-resistant mutants that over-express an Arabidopsis UDP-glycosyltransferase, which likely inactivates FSM by glycosylation. This method is useful for chemical genetics studies in plant biology when inhibitor causes visible phenotype on Arabidopsis.
