Abstract
Artemisia is a large, diverse genus of plants with between 250-400 species belonging to the family asteraceae. A. momiyamae is endemic to Miura peninsula and Inamuragasaki in Japan. Both chemical and genetic information from these plants is quite limited. These plants are presumed to be the variety of A. princeps from morphological characteristics. We have been clustering Artemisia species based on initial closure mechanism from their common substratel. In this study, this method was applied A. momiyamae. Leaves of 33 Artemisia species were collected and analyzed their terpnoid profiles by GC-MS (Refer M. Shimomura's poster). Sesquiterpenoids are separated into four group base on initial closure mechanism from the substrate farnesyl diphosphate. We chose a group of compounds (total 22) biosynthesized through initial 1,6 closure, and clustered 17 Artemisia species using these compounds. Cluster I: Amorpha-4,11-diene and these analogs were included (A. annua). Cluster II: β-sesquiphellandrene and α-zingiberene were common compounds (4 Artemisia species). Especially, sub-cluster was found between A. momiyamae and A. stolonifera because of the presence of bisabolone. Cluster III: Various monocyclic sesquiterpenoids were included (12 Artemisia species). From chemometrics cluster analysis, A. momiyamae is closer to A. stolonifera than to A. princeps.
