Abstract
On the signaling pathway for gibberellin (GA) in plants, the GA-dependent GID1-DELLA interaction is a main event for its transduction. After their interaction, the GID1-bound DELLA shows an affinity to a GA-specific F-box protein, which is a member of E3 ubiquitin ligase complex. In Arabidopsis, SLY1 is known as the protein for GA signaling. We had already established the yeast three hybrid system (Y3H) consisted from GID1, DELLA, and SLY1. By using this system, we can monitor the SLY1's activity to receive a DELLA molecule from the GID1-DELLA complex. In this report, we apply this Y3H system to screen inhibitors to the SLY1's activity by using commercially available chemical library. We selected 21 chemicals as candidates, and now in planta evaluations are proceeding.
