Abstract
A novel calorimetric flow-injection analysis for cofactors with use of immobilized metalloenzymes and thermistors was proposed. The biosensing system was assembled with a combination of the enzyme-reactor and the thermistor probe for monitoring the enzymatic activity. Heavy metal ions ranging from micromolar to millimolar levels were calorimetrically determined through its activation of the immobilized metal-free enzyme (apoenzyme) reactor. The overview of the apoenzyme reactivation microassay for heavy metal ions was described.
