Abstract
The Terumo TAF08W (regenerated cellulose) with a higher cut-off point than the conventional type, the Terumo TE10W and the Asahi Neo-10 (cuprophan) were used on patients with disequilibrium syndrome over a three-monthperiod. Changes in clinical condition in the course of hemodialysis treatment were observed and the ultrafiltrate obtained from the dialyzers was analyzed by Sephadex G-15 gel column chromatography, SDS-Polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (TDE). Disequilibrium symptoms such as a drop in blood pressure during hemodialysis treatment were improved by introduction of the regenerated cellulose membrane. No differences in the clearances of low-molecular-weight substances could be observed in the dialyzer. However, the TAF08W showed greater removal of the highest molecular-weight fraction and middle molecular-weight substances compared with the other two dialyzers. In the TAF08W, a little albumin and β2-microglobulin were detected by TDE and several bands of low-molecular-weight protein were apparent by SDS-PAGE, these proteins not being detected in the other two dialyzers.
