Abstract
Conditioned media (CM) of PHA-stimulated lymphocytes from chronic uremic patients were tested for colony-stimulating activities, using methylcellulose hematopoietic progenitor cultures with normal bone marrow cells as a target population.
CMs from uremic patients undergoing hemodialysis were shown to lack potential to support the full growth of early erythroid progenitors (BFU-E) due to the presence of suppressive factors or poor production of burst promoting activity (BPA), or both. However, the stimulatory activity for CFU-GM was increased, particularly among patients on hemodialysis. Disappearance of anti-BPA activities preceded the normalization of hemoglobin levels after the termination of hemodialysis by kidney transplantation.
Based upon the results, disturbance of the regulation of erythropoiesis through leukocyte-derived factor (s) may play a part in the development of anemia in chronic uremic patients.
